The Effects of Different Auxin–Cytokinin Combinations on Morphogenesis of Fritillaria meleagris Using Bulb Scale Sections In Vitro

Fritillaria meleagris is a horticulturally and medicinally valuable bulbous plant that requires period of low temperatures for proper growth flowering. Since conventional methods propagation are ineffective very slow, tissue culture techniques offer an integrated approach to mass production this geophyte. In study, we investigated the effects various auxin–cytokinin combinations on different morphogenetic pathways in bulb scale culture. Bulbs obtained vitro were cut longitudinally, scales cultured four weeks at 7 °C MS medium supplemented with 6-benzylaminopurine (BAP) combination 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic (NAA) concentrations order investigate influence regulators (PGRs) responses. Regeneration percentage, number shoots per explant, shoot length, bulbs somatic embryos monitored weekly. After chilling, transferred 24 °C, all parameters recorded again. Low PGR effective multiplication, yielding up 5.5 explant. 2,4-D (at 2 mg/L) BAP (0.25 produced highest (11.00 ± 0.00), while PGR-free was extremely embryo formation (13.50 2.90). Detached bulblets continued grow develop fresh medium. We present data demonstrating provide method combined pathway F. suitable large-scale propagation.